We observed exocytosis of glutamate receptors around the post-synaptic membrane with total internal reflection fluorescence microscopy. Cultured fat neurons were transfected with GluA1-SEP, glutamate receptor tagged by pH sensitive green fluorescent protein, super ecliptic pHluorin (SEP), and Homer 1C-RFP (a post-synaptic marker). SEP shows fluorescence signals under neutral pH conditions such as an extracellular solution