Exocytosis of glutamate receptors around post-synaptic membrane

Exocytosis of glutamate receptors around post-synaptic membrane

Biophysics NeuroScience PNS001


We observed exocytosis of glutamate receptors around the post-synaptic membrane with total internal reflection fluorescence microscopy. Cultured fat neurons were transfected with GluA1-SEP, glutamate receptor tagged by pH sensitive green fluorescent protein, super ecliptic pHluorin (SEP), and Homer 1C-RFP (a post-synaptic marker). SEP shows fluorescence signals under neutral pH conditions such as an extracellular solution; these signals disappear under acidic conditions such as the internal lumen of vesicles. It was therefore used as an exocytosis marker. Arrows and arrowheads indicate exocytosis around and out of the post-synaptic membrane. Images were acquired every second, and are played at 33 times normal speed. Scale bar is 5 µm.



Prefecture Kyoto Use of Microscope Use
Region Yoshida Campus, Kyoto University Use of Automatic Shooting Devices Don't Use
Shooting Date 2012/8/20 Use of Fluorescent Probe Use
Shooting Time 23:00 Use of Infrared Don't Use
Photographer Hiromitsu TANAKA Shooting Interval (Sec.) 1秒
Copyright Holder Hiromitsu TANAKA Shooting Speed 33倍速

In Japanese